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Pharmaceutical and Biotechnology Drug Discovery, Diagnostic Research Consultant

Technical Consultant #2365


Expertise

  • Pharmaceutical and Biotechnology drug discovery, diagnostic research and development, business development.
  • Identify and implement technologies in strategically relevant areas to maximize value; translate completed research and development into company growth.
  • Small molecule and antibody drug discovery and development.
  • Diagnostic development: solution-based; lateral flow assay development, optimization, and construction; high-throughput screening platforms; assay automation (fluorescence/absorbance, liquid handling robotics).
  • Aptamer selection and characterization.
  • CRISPR/Cas9: Gene editing in therapeutic cell lines. Gene repair/correction of endogenous protein production.
  • Antibody screening and production for human and livestock diagnostic assay development; nucleic acid isothermal amplification method optimization.

Molecular Biology

  • Nucleic acid methodologies: RNA/DNA isolation and purification, real-time PCR, cloning and mutagenesis, in vitro transcription (IVT), isothermal nucleic acid amplification, RNAi knockdown; CRISPR/Cas9 gene-editing methodologies; nucleofection.

Protein Biochemistry

  • Protein purification (IMAC) and expression, gel electrophoresis, in vitro (directed) evolution, protein kinase assays; Antibody-based technologies: western blotting, ELISA, immunoprecipitation; gel shift assay ( /-radioisotopes), S-35 metabolic labeling, fluorescence microscopy.

Cell Culture, Model Organism Handling

  • S2 Drosophila embryonic cell culture, E. coli cell culture for recombinant protein. production.
  • C. elegans, S. cerevisiae, D. melanogaster, human primary cells and cancer cell lines.

Experience

Undisclosed Company, Owner, 2015 - Present

  • Provide expert consultation on strategic planning and implementation of scientific methods to maximize resources and deliver results.
  • Gene editing: CRISPR/Cas9-facilitated gene editing of hemophilia genes in therapeutic cell lines.
  • Successfully demonstrated gene repair that resulted in corrected endogenous protein production; Coordinated on-site and remote training of research staff in wet lab techniques.
  • Resolved technical issues; Conserved financial expenditures (saved nearly $100K) by devising economical methodology and refocusing research efforts towards milestones that translated more readily into value for investors.
  • Antibody production and screening for human and livestock diagnostic assay development, and nucleic acid isothermal amplification method optimization.

Undisclosed Company, Owner Founder and Scientific Director, 2014 - Present

  • Founder of a drug discovery and development company to capitalize on fast-moving niche market for therapeutic indications in oncology.
  • Engineering first-in-class nucleic acid aptamers for the treatment of lung and breast carcinomas.
  • Chemotherapeutic regimens; addressing sensitivity and specificity over current and less-specific.
  • Creating novel research technologies in support of drug discovery activities, including identifying platform technology, negotiating scientific out-licensing and collaboration agreements, and %u2022 orchestrating strategic partnering.

AB, LLC, Co-founder and Scientific Director, 2008 - 2014

  • Founded in vitro diagnostic company to utilize nucleic acid aptamers for detection of protein analytes.
  • Characterized and optimized a combined aptamer-as-detection-molecule and signal readout system to detect target protein and relay the results to the end-user.
  • Provided leadership for USPTO patent development (granted International patent pending).
  • Designed protocols in-house; managed projects with CMOs and CROs.
  • Oversaw all administrative and technical aspects of company, including facility construction, management, and personnel supervision.

Research

Caltech, Biochemistry. Pasadena, CA, 2001 - 2008

Graduate Student Researcher, 2002 - 2008

  • Characterized regulatory motifs in chaperones during early cell development.
  • Elucidated direct protein-protein interaction between cancer/aging and chaperone-associated pathways.

Research Assistant, Computer Science, 2001 - 2002

  • Designed and tested DNA-based transcriptional switches for in vitro computation.
  • Trained Caltech students in molecular biology and nucleic acid wet lab methods.
  • Re-designed laboratory space for new faculty.

University of Virginia, Biology, Research Assistant, 2001

  • Identified primary crystal component in Paramecium tetraurelia.

NASA Jet Propulsion Lab., Pasadena, CA, Internship, 1999 - 2000

Planetary Protection Division

  • Developed spacecraft sterilization techniques for Martian rovers with team of chemists and microbiologists.

Jet Propulsion Lab, Astrobiology Division

  • Characterized chemotaxis (nutrient-sensing) mechanism of prokaryote Shewanella putrefaciens MR-1.

Georgetown University Medical School, Washington, D.C., 1998 - 1999

Research Assistant - Pharmacology

  • Conducted primary statistical analysis of pharmacokinetics/dynamics of malaria drug Halofantrine hydrochloride in direct collaboration with division director; extensive use of Excel for compiling and charting data from patient samples.

National Zoological Park, Genetics Division. Washington, D.C., Internship, 1997

  • Examined mosquito inter-breeding in genus Culex using molecular biology (PCR, cloning) methods at zoo-based lab facility.

Educator

Caltech, Biochemistry. Pasadena, CA, 2002 -2006

  • Instructor: Lecture and Seminar courses - Biology and Chemistry

Honors & Publications


Credentials

  • Current knowledge of intellectual property and patent drafting guidelines.
  • Software: Mfold/NUPACK nucleic acid modeling programs.

Academic and Professional Affiliations

  • Intel International Science and Engineering Fair - Judge and Co-Chair
  • California State Science Fair - Biochemistry - Judges Advisory Committee

Publications and Patents

  • Co-Author of abstracts and peer reviewed publications
  • U.S. and International Patent

Education

  • Ph.D. Biochemistry and Molecular Biophysics, California Institute of Technology, Pasadena, CA
  • M.S. Biochemistry and Molecular Biophysics, California Institute of Technology, Pasadena, CA
  • B.A. Biology, University of Virginia, Charlottesville, VA
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