- Culture of established cell lines (attached or in suspension) for production of biologicals, such as CHO cells and hybridomas.
- Design and optimization of cell culture media with emphasis on the development of animal component-free formulations for biopharmaceutical applications.
- Scale up and process optimization of mammalian cell cultures from research and development to pilot production.
- Application of design of experiment software to the development, scale up and optimization of cell culture processes for increased productivity and reduced costs.
- Research and development of innovative cell culture products manufactured under current Good Manufacturing Practice (cGMP) for use in research and biopharmaceutical applications.
Expertise in morphological and histological procedures
- Organ and tissue dissection, especially in small mammals.
- Tissue processing, i.e. section preparation for microscopy and staining.
- Microscopic examination and interpretation, including electron microscopy.
Contract Research Services
- Biotechnology, contract research and laboratory services.
- Contract research, cell culture assays, immunoassays.
- Testing services, protein analysis culture optimization, flow cytometry.
Undisclosed Company, Founder & Co-Partner, Arizona, 2001 - Present
- Offers consulting services in mammalian cell culture, with emphasis on media design and optimization.
- Uses statistical tools, i.e. design of experiment (DOE) software for screening tests, media design and scale up studies for better results and reduced costs.
- Presently assisting a Brazilian University establish a cell culture program.
Becton-Dickinson, Maryland, Program Manager, Cell Culture, 2000-2001
- Established and directed a new cell culture program.
- Implemented and managed the development and commercialization of proprietary cell culture formulations for industrial production of antibodies.
- Successfully solved performance and manufacturing issues of a serum-free hybridoma formulation, releasing product from back orders.
- Provided technical expertise to company management and recommended strategic areas and technologies for the cell culture industrial market.
Sigma-Aldrich, Manager, Missouri, Cell Biology and Cell Culture, 1997 - 1999
- Led team to launch successfully the first line of cell biology products manufactured in house, which included test kits for the neuroscience market.
- Implemented management tools to better monitor product development in R&D and product transfer to manufacturing.
- Created a cell culture team and developed a new line of cytogenetic products and serum-free cell culture media for vaccine production. Led team to successful launch five new products for in vitro diagnostics.
- Supervised the development and release of four new serum-free formulations in a kit format, optimized in bioreactors for use in veterinary vaccine production.
- Coordinated the research and development activities of a cell culture team and doubled product output by updating design protocols and increasing team performance.
BioWhittaker, Inc., Maryland, Manager, Cell Culture, 1992 - 1997
- Coordinated the release of fifteen new products for application in the biopharmaceutical and clinical diagnostic markets including:
- Serum free formulations cell lines used in the production of antibodies, recombinant proteins and viral vaccines.
- FDA (510k) approved transport medium for clinical specimens.
- Formulation for growth of amniocytes.
- Improved serum free culture medium for human keratinocytes with increased stability and lower protein content.
- Implemented microfluidics technology for in-house production of lipid solutions.
- Designed and implemented the company's new cell culture facility.
University of Texas Southwestern Medical Center, Dallas, Research Fellow, 1990 - 1992
- Developed a cell culture system for transfection of normal human mammary cells with SV40 large T antigen and E6/E7 papilloma virus.
- Examined the mechanisms of senescence and analyzed the frequency of immortalization.
- Examined the karyotype of immortal clones and demonstrated less constraint in the immortalization of epithelial cells.
Federal University of Sergipe, Brazil, Associate Professor of Cell Biology and Vice-Chairman, Department of Morphology, 1986 - 1990
- Studied the role of matrix glycoproteins in regulating fetal growth of the mammary gland.
- Implemented a database system to correlate patient clinical history, mammographic patterns and risks for breast cancer.
- Coordinated the implementation of a new Cell Biology curriculum.
University of Colorado Health Sciences Center, Denver, Post-Doctoral Fellow, Department of Pathology, 1984 - 1986
- Developed organotypic cultures as in vitro models for studies of stromal-epithelial interactions during growth and differentiation of fetal and transformed mammary cells.
- Demonstrated the generation of carcinogenic metabolites by mammary stromal cells in vitro.
Honors & Publications
Academic and Professional Affiliations
- Arizona BioIndustry Association
- Research Fellowship, Susan Komen Breast Cancer Foundation
- Research Grant, Brazilian Research Council (CNPq)
- Post-Doctoral Training, Department of Pathology, University of Colorado Health Sciences Center, Denver.
- Medical Residency in Pathology, University Hospital, Federal University, Bahia, Brazil.
- Instructor of Histology and Pathology, Brazil.
- Fluent and excellent writing skills in English, Portuguese and French.
- Over one dozen publications and articles written, and over 20 presentations given.
- Ph.D. Cellular Biology, University of Colorado, Boulder, CO
- M.D. School of Medicine, Federal University, Bahia, Brazil
- M.A. Biological Sciences, University of Missouri, Columbia, MO