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Cell Culture Consultant: Breast Cancer Research, Human Epithelial Cells, Endothelial Cells, Stem Cells and Production of Mammalian Cells

Technical Consultant #1524


  • Culture of established cell lines (attached or in suspension) for production of biologicals, such as CHO cells and hybridomas.
  • Design and optimization of cell culture media with emphasis on the development of animal component-free formulations for biopharmaceutical applications.
  • Scale up and process optimization of mammalian cell cultures from research and development to pilot production.
  • Application of design of experiment software to the development, scale up and optimization of cell culture processes for increased productivity and reduced costs.
  • Research and development of innovative cell culture products manufactured under current Good Manufacturing Practice (cGMP) for use in research and biopharmaceutical applications.

Expertise in morphological and histological procedures

  • Organ and tissue dissection, especially in small mammals.
  • Tissue processing, i.e. section preparation for microscopy and staining.
  • Microscopic examination and interpretation, including electron microscopy.

Contract Research Services

  • Biotechnology, contract research and laboratory services.
  • Contract research, cell culture assays, immunoassays.
  • Testing services, protein analysis culture optimization, flow cytometry.


Undisclosed Company, Founder & Co-Partner, Arizona, 2001 - Present

  • Offers consulting services in mammalian cell culture, with emphasis on media design and optimization.
  • Uses statistical tools, i.e. design of experiment (DOE) software for screening tests, media design and scale up studies for better results and reduced costs.
  • Presently assisting a Brazilian University establish a cell culture program.

Becton-Dickinson, Maryland, Program Manager, Cell Culture, 2000-2001

  • Established and directed a new cell culture program.
  • Implemented and managed the development and commercialization of proprietary cell culture formulations for industrial production of antibodies.
  • Successfully solved performance and manufacturing issues of a serum-free hybridoma formulation, releasing product from back orders.
  • Provided technical expertise to company management and recommended strategic areas and technologies for the cell culture industrial market.

Sigma-Aldrich, Manager, Missouri, Cell Biology and Cell Culture, 1997 - 1999

  • Led team to launch successfully the first line of cell biology products manufactured in house, which included test kits for the neuroscience market.
  • Implemented management tools to better monitor product development in R&D and product transfer to manufacturing.
  • Created a cell culture team and developed a new line of cytogenetic products and serum-free cell culture media for vaccine production. Led team to successful launch five new products for in vitro diagnostics.
  • Supervised the development and release of four new serum-free formulations in a kit format, optimized in bioreactors for use in veterinary vaccine production.
  • Coordinated the research and development activities of a cell culture team and doubled product output by updating design protocols and increasing team performance.

BioWhittaker, Inc., Maryland, Manager, Cell Culture, 1992 - 1997

  • Coordinated the release of fifteen new products for application in the biopharmaceutical and clinical diagnostic markets including:
  • Serum free formulations cell lines used in the production of antibodies, recombinant proteins and viral vaccines.
  • FDA (510k) approved transport medium for clinical specimens.
  • Formulation for growth of amniocytes.
  • Improved serum free culture medium for human keratinocytes with increased stability and lower protein content.
  • Implemented microfluidics technology for in-house production of lipid solutions.
  • Designed and implemented the company's new cell culture facility.

University of Texas Southwestern Medical Center, Dallas, Research Fellow, 1990 - 1992

  • Developed a cell culture system for transfection of normal human mammary cells with SV40 large T antigen and E6/E7 papilloma virus.
  • Examined the mechanisms of senescence and analyzed the frequency of immortalization.
  • Examined the karyotype of immortal clones and demonstrated less constraint in the immortalization of epithelial cells.

Federal University of Sergipe, Brazil, Associate Professor of Cell Biology and Vice-Chairman, Department of Morphology, 1986 - 1990

  • Studied the role of matrix glycoproteins in regulating fetal growth of the mammary gland.
  • Implemented a database system to correlate patient clinical history, mammographic patterns and risks for breast cancer.
  • Coordinated the implementation of a new Cell Biology curriculum.

University of Colorado Health Sciences Center, Denver, Post-Doctoral Fellow, Department of Pathology, 1984 - 1986

  • Developed organotypic cultures as in vitro models for studies of stromal-epithelial interactions during growth and differentiation of fetal and transformed mammary cells.
  • Demonstrated the generation of carcinogenic metabolites by mammary stromal cells in vitro.

Honors & Publications

Academic and Professional Affiliations

  • Arizona BioIndustry Association
  • Research Fellowship, Susan Komen Breast Cancer Foundation
  • Research Grant, Brazilian Research Council (CNPq)


  • Post-Doctoral Training, Department of Pathology, University of Colorado Health Sciences Center, Denver.
  • Medical Residency in Pathology, University Hospital, Federal University, Bahia, Brazil.
  • Instructor of Histology and Pathology, Brazil.


  • Fluent and excellent writing skills in English, Portuguese and French.
  • Over one dozen publications and articles written, and over 20 presentations given.


  • Ph.D. Cellular Biology, University of Colorado, Boulder, CO
  • M.D. School of Medicine, Federal University, Bahia, Brazil
  • M.A. Biological Sciences, University of Missouri, Columbia, MO
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