- Drug Discovery: Designed, developed, patented and licensed novel compounds for treatment of multiple sclerosis which are being tested in clinical trials.
- Cell and Organ Culture: Primary cells, cell lines and clones, hybridomas, L-transfectants.
- Cell Manipulations: Isolation and purification of various cell types from mouse and human tissues including T cells, B cells, monocytes, macrophages, dendritic cells, and neutrophils.
- Cellular Assays: Design and development of cell-based assays for screening of candidate drug compounds (T cell activation, proliferation; antigen presentation, cytokine secretion, chemotaxis).
- Flow Cytometry: Proficiency in multicolour FACS analysis, sample preparation and staining; FACS-based assays (apoptosis, BrDU incorporation, intracellular cytokine staining), cell sorting.
- Protein Work: Purification of antibodies, HLA proteins; preparation of tetramers; design of peptides and copolymers; development of protein - based assays for drug screening; Western blot.
- Molecular Biology: Purification of DNA and RNA; restriction enzyme digestions; agarose and polyacrylamide gel electrophoresis; PCR; in situ hybridization; Southern blot.
- Animal Work: Establishment of autoimmune models for screening of candidate drug compounds; generation of irradiation chimeras; injections (intraocular, intra - peritoneal, subcutaneous); harvesting of organs; ex-vivo cellular assays.
Independent Pharmaceutical Consultant, 2006-Present
Introduced and established immunological approaches for drug screening assays:
- Purchased and operated FACSCalibur flow cytometer.
- Performed multicolor immunofluorescence cell staining, acquisition and data analysis.
- Developed FACS-based assay for measuring of reactive oxygen species production in neutrophils isolated from peripheral blood and in cell lines.
- Established cell isolation protocols using magnetic bead immunolabeling methods.
- Set-up cytokine secretion assay in primary cells using intracellular staining procedure.
- Introduced a cellular screening assay detecting pro - inflammatory cytokines by cell surface immunostaining approach.
- Established in-house assay for transmigration of neutrophils and monocytes.
- Measured cytokine secretion in endothelial and glial cells as targets for anti-inflammatory compounds.
- Advised on the in vivo models of inflammation and autoimmunity as candidate systems for drug screening.
Dana Farber Cancer Institute, Boston, MA, Visiting Scientist, 2005-Present
Research Project: Genetic associations between autoimmune and infectious diseases.
- Designed functional peripheral blood cell-based assays for measuring lymphocyte proliferation and cytokine secretion in rheumatoid arthritis patients developing tuberculosis upon treatment with Enbrel.
- Taught selected topics on T cell development, aging, autoimmunity and immunotherapies to undergraduate and graduate students at Harvard Medical School.
Molecular Immunology Foundation, Boston, MA, Principal Investigator, 2001-2005
- Developed transgenic mouse models and irradiation chimeras for studies of T cell maturation and emigration.
- Designed peptide - based compounds for thymic vaccination.
- Collected and analyzed tissues by biochemical and immunological methods.
- Established experimental in vitro systems and functional assays for T cell positive selection, proliferation and immigration.
- Employed immunofluorescent methods and FACS analysis for cell phenotyping.
- Produced HLA class I/peptide tetramers for detection of rare T cell populations.
Harvard University, Cambridge, MA, Research Associate, 1995-2001
Department of Molecular and Cellular Biology
Research Project: Development of novel therapies for autoimmune diseases.
- Studied the MoA of Copaxone as a basis for development of novel therapies.
- Designed peptide and protein based compounds for treatment of multiple sclerosis and rheumatoid arthritis.
- Used animal models of these diseases and established in vivo protocols for administration of tested compositions.
- Studied protein-protein interactions, T cell responses and cytokine secretion profiles.
Research Project: Synthetic Copolymers and Peptides as Candidate Therapies for Autoimmune Inflammatory Diseases.
- Developed in vitro ELISA based assays for detection of protein-ligand interactions in the context of Copaxone binding to HLA class II molecules.
- Handled large scale production of monoclonal antibodies for immunoaffinity purification of HLA-DR proteins.
- Purified membrane and recombinant HLA-DR proteins from EBV - transformed homozygous B cell lines and insect cells, respectively.
- Developed cell-based assays for screening of peptides and copolymers.
- Employed immunofluorescent methods and FACS analysis.
Weizmann Institute of Science, Rehovot, Israel, Post-doctoral Fellow, Department of Immunology, 1992-1995
Research Project: Binding of Copolymer 1 to MHC Molecules on Antigen-Presenting Cells.
- Developed a fluorescent labeling method for detection of Copaxone on the surface of living antigen-presenting cells.
- Established cellular assays for binding of Copaxone and peptides to the surface HLA receptors.
- Used various tissue culture techniques.
- Implemented cell staining methods and flow cytometry for detecting of peptide/MHC or Copaxone/MHC complexes.
Honors & Publications
- Proficiency in three languages: English, Hebrew and Russian
- Two issued patents and twenty pending patent applications
- Thirty-eight publications
- Invited speaker at universities and biotech companies worldwide
- Member, Society of Industry Leaders
- Ph.D., Cell Biology and Immunology, Weizmann Institute of Science, Rehovot, Israel
- M.Sc., Molecular Genetics, Hebrew University, Jerusalem, Israel
- B.A., Biology, Technion-Israel Institute of Technology, Haifa, Israel