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Protein Biophysics Consultant: Protein Identification and Analysis

Technical Consultant #1317


Expertise

  • Identification of post-translational protein modifications (phosphoproteins)
  • Peptide mapping and protein ID by LC/MS/MS
  • Enzymology
  • Analytical ultracentrifugation
  • Mass spec analysis of proteins, peptides, organic molecules by LC/MS
  • Automated protein purification and electrophoresis
  • Calorimetry
  • HPLC
  • Immunological Techniques

Experience

Merck & Co., West Point, PA 1986-2005

Senior Research Fellow (1994-2005)

  • Evaluated new protein analytical platforms and performed protein analysis for all proteins submitted for x-ray crystallography.
  • Purified recombinant proteins for HCV, CMV, HIV programs.
  • Set up assays for proteases, kinases, and viral enzymes.
  • Determined solubility properties of all compounds slated for structural studies.
  • Set-up protein analysis group within Department of Structural Biology improving consistency and timeliness of data to scientists; 2rote SOPs for protein sample submission, use of instruments, and analysis of data.
  • Introduced and implemented mass spectrophotometry, light scattering, Thermofluor, high-throughput mass spec analysis of proteins improving protein analysis and quality control.
  • Implemented techniques to purify and crystallize membrane proteins.
  • Introduced limited proteolysis to identify functional domains in drug targets such as Kinases.
  • Active member of Research Technologies Review & Licensing Committee.

Research Fellow (1986-1993)

  • Focused on purifying proteins for various research programs; developed assays for viral proteases and exploratory cancer targets.
  • Developed assays for inhibitors of blood coagulation.
  • Set-up screen and counter screens for endothelin converting enzymes.
  • Identified and purified novel anticoagulant proteins from ticks and hookworms.
  • Purified and characterized retroviral protease from avian myeloblastosis virus.
  • Purified and characterized membrane-bound endothelin converting enzyme from cultured cells.
  • Identified sites of cleavage in p55 GAG by HIV protease.

Harvard Medical School, Boston, MA 1980-1986

Senior Research Associate

  • Oversaw daily laboratory management, trained new team members, and wrote grants.
  • Studied Proteases and Protein breakdown, Laboratory Alfred L. Goldberg, Department of Physiology and Biophysics.

University of California, Davis, CA 1976-1979

Research Associate and Lecturer (1977-1979)

  • Carried out structural analysis of protein kinases, Laboratory of Donal A. Walsh, Department of Biological Chemistry. Supported by fellowship from NIH.

Postdoctoral Fellow (1976-1977)

  • Purified and characterized protein kinases, Laboratory of Edwin G. Krebs, Department of Biological Chemistry. Supported by fellowship from Muscular Dystrophy Association.

Harvard University, Cambridge, MA 1974-1976

Postdoctoral Fellow

  • Analyzed phoshoproteins in red blood cell membranes, Laboratory of Dr. Guido Guidotti, Department of Biochemistry. Supported by fellowship from American Leukemia Society.

Academic Work Experience

  • Lecturer in Biochemistry, U.C. Davis Medical School, Davis, CA
  • Teaching Fellow in Biochemistry, Harvard University, Cambridge, MA
  • Teaching Fellow in Biology, Harvard University, Cambridge, MA
  • Research Assistant in Physics, Temple University, Philadelphia, PA
  • Undergraduate Teaching Assistant in Physics, Temple University, Philadelphia, PA

Honors & Publications


Twenty-five publications

Fur patents


Education

PhD., Biophysics, Harvard University, Cambridge, MA

M.A., Physics, Temple University, Philadelphia, PA

B.A., Physics, Temple University, Philadelphia, PA

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