Molecular Biology Consultant: Genetics, Oncology, Endocrinology, Estrogens, Pharmacology and Expert Witness

Technical Consultant #1528


Expertise

  • Pharmacology: Mechanisms of drug action and side-effects.
  • Oncology: Specializing in breast cancer, endocrine regulated cancers and lung cancer.
  • Endocrinology: Hormone replacement therapy with estrogens and progestins.
  • Steroid receptors, steroid hormones and mechanisms of action.
  • Dose-response curves: Tamoxifen, raloxifene, fulvestrant, SERMs, letrozole, anastrozole, exemestane and aromatase inhibitors.
  • Molecular biology, biochemistry, biophysics.
  • Recombinant DNA technology, PCR, mutagenesis, cloning, DNA sequence analysis and hybridization blots.
  • Gene expression: Protein production, translation, purification, phosphorylation and immunobloting.
  • Transcription: RNA polymerases, transfer RNAs, RNA processing, RNA interference and microRNAs.
  • Assay development - reporter assays with luciferase and green fluorescent protein GFP.
  • In vitro mammalian cell culture: immunohistochemistry IHC, tissue preparation and human histology.
  • Genetics-cancer genetics and pharmacogenetics.
  • Microbiology: E. coli, and yeasts S. cerevisiae and S. pombe.
  • Genetic screens in yeasts S. cerevisiae and S. pombe and %u03B2-galactosidase.
  • Teaching medicine and medical topics; adept at researching complex topics, simplifying them, and teaching others.
  • Pharmaceutical drug development, safety and patent protection.
  • Interpreting expert in scientific literature.
  • Expert witness: Pharmacology, oncology, endocrinology and molecular biology: estrogens, cancers, pharmaceuticals and RNA interference.

Experience

Undisclosed University, Assistant Professor of Pharmacology, 1998 - present

  • Focus in molecular biology, pharmacology, endocrinology and oncology medical research.
  • Director and manager of academic medical research lab and projects on steroid hormone receptors for 16 years, primarily the estrogen receptors alpha and beta (ER%u03B1, ER%u03B2) and their roles in normal and in cancer tissue.
  • Detailed structure-function analysis of: (a) transfer RNAs and (b) estrogen receptor hormone binding domain through the use of mutagenesis and subsequent assays for function.
  • Skilled in ligand-binding and DNA-binding assays and numerous others.
  • Developing technology for discovery of receptor-subtype-selective ligands for ER%u03B1 vs.ER%u03B2 and independently, to detect anabolic steroids in samples.
  • Authored and presented lectures, workshops, conferences for Ph.D. and M.D. students on a very wide variety of medical and scientific topics of 15 years.

Undisclosed Company, Legal Consultant, 2005 - Present

  • Expert witness in a patent dispute over a generic drug application. Patent lawsuit centered on the use and novelty of an aromatase inhibitor that blocks estrogen production. Work included detailed expert report and videotaped deposition.
  • Expert witness on litigation related to the potential role of estrogen in a lung cancer patient case; consultant on the roles of estrogens and progestins in breast cancer patients who took hormone replacement therapy (HRT).

European Molecular Biology Lab (EMBL), Heidelberg, Germany, Visiting Researcher, 1992 - 1998

  • Led projects to find important amino acids for human Estrogen Receptor function, using extensive in vitro mutagenesis and fusions to site-specific recombinases (Cre, FLP) in a novel yeast genetic color and growth assay.
  • Optimized 32P radiolabeled RNA probes for use in hybridization blotting for very high sensitivity.
  • Expert use of phosphorimager to quantify signals.
  • Developed unique yeast plate assay to report estrogen binding as an inherited genetic change.

German Cancer Research Center (DKFZ), Heidelberg, Germany, 1988 - 1992

  • Post Doctoral Fellow-EMBO Fellowship
  • Focused on mammalian RNA polymerase II transcription and characterization of proteins that interact at a tissue specific and hormone inducible enhancer sequence.
  • Made nuclear and cytoplasmic protein extracts from liver tissue.
  • Initiated purchase and successful use of FPLC protein purification equipment.
  • Expert in running 2-D protein gels and studying phosphorylation patterns on a transcription factor.
  • Set up and utilized band-shift experiments to study protein-DNA interactions.

Yale University, New Haven, CT, Molecular Biophysics & Biochemistry, 1981 - 1987

  • Studied transfer RNA synthesis, RNA processing, RNA modification, RNA Polymerase III transcription and protein purification, suppressor tRNAs, genetic screens and molecular biology in the baker's yeast, S. cerevisiae.
  • First to perform oligonucleotide-mediated site-specific mutagenesis of a gene.
  • Performed yeast extract preparation and 32P radiolabeled transcription assays.
  • Initiated protein purification techniques in the lab.
  • Utilized new software to study tRNA sequence alignments.
  • Optimized a rapid and graded color screen for tRNA function.

Honors & Publications

  • Recipient of Prestigious Breast Cancer Research Award
  • Fellow in Innovative Cancer Research
  • University Innovator Award (Licensed IP to Industry)

Academic and Professional Affiliations

  • Completed course on academic intellectual property transfer, From Basic Research to Therapeutic Use: What Every Scientist Needs to Know.
  • Scientific Advisory Board Member, Undisclosed Firm
  • Genetics Society of America GSA
  • The Endocrine Society
  • American Association for Cancer Research, AACR

Publications

  • Numerous Publications and Patents
  • Inventor of patented technology in RNA interference.

Education

  • Ph.D. Molecular Biophysics and Biochemistry, Yale University, New Haven, CT
  • M.A. Biochemistry, University of California, Berkeley, CA
  • MBA Biomedical Scientists, University of Pittsburgh, PA
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