- Pharmacology: Mechanisms of drug action and side-effects.
- Oncology: Specializing in breast cancer, endocrine regulated cancers and lung cancer.
- Endocrinology: Hormone replacement therapy with estrogens and progestins.
- Steroid receptors, steroid hormones and mechanisms of action.
- Dose-response curves: Tamoxifen, raloxifene, fulvestrant, SERMs, letrozole, anastrozole, exemestane and aromatase inhibitors.
- Molecular biology, biochemistry, biophysics.
- Recombinant DNA technology, PCR, mutagenesis, cloning, DNA sequence analysis and hybridization blots.
- Gene expression: Protein production, translation, purification, phosphorylation and immunobloting.
- Transcription: RNA polymerases, transfer RNAs, RNA processing, RNA interference and microRNAs.
- Assay development - reporter assays with luciferase and green fluorescent protein GFP.
- In vitro mammalian cell culture: immunohistochemistry IHC, tissue preparation and human histology.
- Genetics-cancer genetics and pharmacogenetics.
- Microbiology: E. coli, and yeasts S. cerevisiae and S. pombe.
- Genetic screens in yeasts S. cerevisiae and S. pombe and %u03B2-galactosidase.
- Teaching medicine and medical topics; adept at researching complex topics, simplifying them, and teaching others.
- Pharmaceutical drug development, safety and patent protection.
- Interpreting expert in scientific literature.
- Expert witness: Pharmacology, oncology, endocrinology and molecular biology: estrogens, cancers, pharmaceuticals and RNA interference.
Undisclosed University, Assistant Professor of Pharmacology, 1998 - present
- Focus in molecular biology, pharmacology, endocrinology and oncology medical research.
- Director and manager of academic medical research lab and projects on steroid hormone receptors for 16 years, primarily the estrogen receptors alpha and beta (ER%u03B1, ER%u03B2) and their roles in normal and in cancer tissue.
- Detailed structure-function analysis of: (a) transfer RNAs and (b) estrogen receptor hormone binding domain through the use of mutagenesis and subsequent assays for function.
- Skilled in ligand-binding and DNA-binding assays and numerous others.
- Developing technology for discovery of receptor-subtype-selective ligands for ER%u03B1 vs.ER%u03B2 and independently, to detect anabolic steroids in samples.
- Authored and presented lectures, workshops, conferences for Ph.D. and M.D. students on a very wide variety of medical and scientific topics of 15 years.
Undisclosed Company, Legal Consultant, 2005 - Present
- Expert witness in a patent dispute over a generic drug application. Patent lawsuit centered on the use and novelty of an aromatase inhibitor that blocks estrogen production. Work included detailed expert report and videotaped deposition.
- Expert witness on litigation related to the potential role of estrogen in a lung cancer patient case; consultant on the roles of estrogens and progestins in breast cancer patients who took hormone replacement therapy (HRT).
European Molecular Biology Lab (EMBL), Heidelberg, Germany, Visiting Researcher, 1992 - 1998
- Led projects to find important amino acids for human Estrogen Receptor function, using extensive in vitro mutagenesis and fusions to site-specific recombinases (Cre, FLP) in a novel yeast genetic color and growth assay.
- Optimized 32P radiolabeled RNA probes for use in hybridization blotting for very high sensitivity.
- Expert use of phosphorimager to quantify signals.
- Developed unique yeast plate assay to report estrogen binding as an inherited genetic change.
German Cancer Research Center (DKFZ), Heidelberg, Germany, 1988 - 1992
- Post Doctoral Fellow-EMBO Fellowship
- Focused on mammalian RNA polymerase II transcription and characterization of proteins that interact at a tissue specific and hormone inducible enhancer sequence.
- Made nuclear and cytoplasmic protein extracts from liver tissue.
- Initiated purchase and successful use of FPLC protein purification equipment.
- Expert in running 2-D protein gels and studying phosphorylation patterns on a transcription factor.
- Set up and utilized band-shift experiments to study protein-DNA interactions.
Yale University, New Haven, CT, Molecular Biophysics & Biochemistry, 1981 - 1987
- Studied transfer RNA synthesis, RNA processing, RNA modification, RNA Polymerase III transcription and protein purification, suppressor tRNAs, genetic screens and molecular biology in the baker's yeast, S. cerevisiae.
- First to perform oligonucleotide-mediated site-specific mutagenesis of a gene.
- Performed yeast extract preparation and 32P radiolabeled transcription assays.
- Initiated protein purification techniques in the lab.
- Utilized new software to study tRNA sequence alignments.
- Optimized a rapid and graded color screen for tRNA function.
Honors & Publications
- Recipient of Prestigious Breast Cancer Research Award
- Fellow in Innovative Cancer Research
- University Innovator Award (Licensed IP to Industry)
Academic and Professional Affiliations
- Completed course on academic intellectual property transfer, From Basic Research to Therapeutic Use: What Every Scientist Needs to Know.
- Scientific Advisory Board Member, Undisclosed Firm
- Genetics Society of America GSA
- The Endocrine Society
- American Association for Cancer Research, AACR
- Numerous Publications and Patents
- Inventor of patented technology in RNA interference.
- Ph.D. Molecular Biophysics and Biochemistry, Yale University, New Haven, CT
- M.A. Biochemistry, University of California, Berkeley, CA
- MBA Biomedical Scientists, University of Pittsburgh, PA