Biochemical Consultant for Green-Fluorescent Protein, Bioluminescence and Biochemistry of Luminescent Systems

Technical Consultant #1247


Expertise

  • Green-fluorescent protein (GFP): isolation and characterization
  • Marine bioluminescence
  • Electrophoresis
  • GFP in HTS assay development
  • Biochemistry of luminescent systems
  • Protein and peptide chemistry including isolation and purification
  • Batch methods of protein purification such as tangential flow ultra filtration and three-phase partitioning.
  • Protein photoinactivation
  • HPLC
  • Bioluminescent genetic assay development
  • Protease assay development
  • Assay design and diagnostics kits.

Experience

  • Independent Consultant %u2013 biochemical systems
  • University Associate Professor, Biochemistry, Department of Biochemistry and Microbiology, since 1977
  • Visiting Scientist, Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minn., 1987%u201188
  • Associate Professor Biochemistry, Department of Biochemistry and Microbiology

Honors & Publications

  • Keynote Speaker: Society for Biomolecular Screening, Biotechnology 2001, Bioluminescence. & Chemiluminescence, Symposium on GFP and Aequorin, Bio-Med Symposium
  • Patent: Bioluminescent Indicator Based upon the Expression of a Gene for a Modified Green- Fluorescent Protein and 6 others regarding GFP
  • Publications: more than 100
  • Books published: three (3) on fluorescent proteins and bioluminescence

Education

  • Ph.D., Biology (Biochemistry), Johns Hopkins University
  • M.S. , Zoology, University of Florida
  • B.S. Ed., Zoology, University of Florida
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