Molecular Biology Consultant: Cell-based Assays, High Throughput Screening, Target Identification, Gene Therapy and Design of Cell- and Molecular Biology Laboratories (1410)
Expertise
Consultant on biotechnology lab automation, high throughput screening and biological assay development.
Consultant and expert in design and development of high content cell based assays for high throughput screening.
Development of non-viral gene delivery techniques.
Expertise in virology, primarily retrovirus and adenovirus.
Embryonic stem cell techniques including high Content screening .
Expert and consultant in the areas of automated and regular microscopy and image analysis.
Consultant for laboratory automation and design of custom made cell culture systems.
Teaching and consulting expertise in gene mapping, molecular genetics, applied virology, digital microscopy gene therapy and restriction fragment length polymorphism (or RFLP).
Successful in establishing a fully integrated center for High Through-Put screening in a multi-disciplinary environment with the capacity to screen and analyze up to 15,000,000 samples/year.
Expert and consultant in gene delivery methodology, transfection and transduction.
Expert in Polymerase Chain Reaction (PCR) methodology.
Designed systems for high throughput cloning and production of lenti and adeno virus based systems.
Consultant on onjugation of functional peptides and proteins to nucleic acids.
Expert in establishing transgenic clones.
Consulting in designing GMP compliant gene therapy laboratories automated with high throughput screening technology.
Expert in establishing B-cell lines from both healthy donors and immune-compromised patients.
Expert in murine bone marrow transplantation and murine bone marrow extraction.
Experienced in nuclear injection/transfection both manual and automated modes.
Experience
Associate Director, University Genome Center 2004 - Present
Influences and guides the activities at the Genome Center on an executive level.
Managed to establish University Genome Center with vendors and industry partners to generate a $7,000,000 screening center from a $1,500,000 original investment.
Directly controls and guides biological lab operations and strategic planning and execution of long-term projects.
Planned and executed the formation of a state-of-the-art high throughput screening facility with a staff of 25 scientists.
Director of Automation, University: High Throughput Screening in Cell Based Assays 2004 - Present
Designed and planned the automation suit for hight throughput screen lab, including the in-house designed automated cell culture system capable of 4x10E9 cells per batch.
Postdoctoral Research Fellow: Laboratory of Cellular Biochemistry & Biophysics, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, NY 2002 - 2004
Trouble shooting of a multi-million dollar functional proteomics project aimed at establishing a full genome wide collection of in-house synthesized siRNA's.
Project Manager: Gene delivery, Clinical Research Center, Karolinska Institute 2001 - 2002
Managed a team of 8 scientists working on a project aimed at develop technologies relating to non-viral gene delivery techniques, medical devices for enhanced transfection/transduction and receptor-ligand interactions.
Co-founder of Avaris AB 2001
The driving force behind the incorporation of Avaris AB and established the company based on patents generated during and after PhD work.
Submitted several patents relating to gene transfer and medical devices.
Visiting Scientist, Perkin-Elmer, Cambridge, UK 2001 - 2005
Established protocols for 4-D visualization of real-time apoptosis in FYK-/FYN-/Btk /- cells.
Established protocols for 4-D visualization of the infection by attenuated Poliovirus in actin-GFP transfected human cells.
Visiting scientist at Oswel Scientific, Southampton, UK 2000 - 2005
Established protocols for conjugation of S.M nuclease to Peptide Nucleic Acid (PNA).
Visiting Scientist at Department of Biosciences, Division of Biochemistry, University of Helsinki, Finland 1998 - 2000
Established the result basis for "BTK-base", database describing the mutations in Brutons Tyrosine Kinase, a cytoplasmic tyrosine kinase.
Visiting Scientist at "Division of Research Immunology and Bone Marrow Transplantation" Children's Hospital of Los Angeles with Donald B. Kohn 1998 - 2006
Established SOP's for generation of MMLV producing clones.
Visiting Scientist at Umea University
Established protocols for the characterization of the genomic organization of Brutons Tyrosine kinase, Btk.
Validated genomic phage libraries for exon-intron characterization of the Btk-gene.
Honors & Publications
Patents and Publications
Many publications and invited lectures.
Patents in diverse areas such as gene delivery, medical devices, receptor specificity screening etc.
Memberships
Swedish Medical Society
Swedish Pharmaceutical Society
American Society for Gene Therapy
European Society for Gene Therapy
Royal Institute of Technology, Confocal Microscopy Society
Education
PhD, Karolinska Institute, Sweden, 2001 Cell and Molecular Biology, Thesis, "The development of synthetic gene delivery systems."
MSc, University of Lund, Sweden, 1993, Molecular Genetics
Additional education, PhD or higher level:
Signal transduction
Understanding signal transduction
Understanding eukaryotic gene expression
Experimental animal management
Transgenic mice
Digital microscopy
GFP and Advanced Microscopy in Cell Biology