Biochemical Expert for Green-Fluorescent Protein (1247)
Expertise
Green-fluorescent protein (GFP): isolation and characterization
Marine bioluminescence
Electrophoresis
GFP in HTS assay development
Biochemistry of luminescent systems
Protein and peptide chemistry including isolation and purification
Batch methods of protein purification such as tangential flow ultra filtration and three-phase partitioning.
Protein photoinactivation
HPLC
Bioluminescent genetic assay development
Protease assay development
Assay design and diagnostics kits.
Experience
Independent Consultant – biochemical systems
University Associate Professor, Biochemistry, Department of Biochemistry and Microbiology, since 1977
Visiting Scientist, Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minn., 1987‑88
Associate Professor Biochemistry, Department of Biochemistry and Microbiology
Honors & Publications
Keynote Speaker: Society for Biomolecular Screening, Biotechnology 2001, Bioluminescence. & Chemiluminescence, Symposium on GFP and Aequorin, Bio-Med Symposium
Patent: Bioluminescent Indicator Based upon the Expression of a Gene for a Modified Green- Fluorescent Protein and 6 others regarding GFP
Publications: more than 100
Books published: three (3) on fluorescent proteins and bioluminescence
Education
Ph.D., Biology (Biochemistry), Johns Hopkins University
M.S. , Zoology, University of Florida
B.S. Ed., Zoology, University of Florida